@article{85221,
  keywords = {Animals, Zebrafish, Cell Proliferation, Microscopy, Electron, Scanning, Cilia, Body Fluids},
  author = {Noriko Okabe and Bo Xu and Rebecca Burdine},
  title = {Fluid dynamics in zebrafish Kupffer{\textquoteright}s vesicle.},
  abstract = { Work in mouse has implicated cilia motility and leftward nodal flow as the mechanism for breaking left-right symmetry. In zebrafish, it is assumed that Kupffer{\textquoteright}s vesicle is analogous to the mouse node. However, its architecture is different and the fluid dynamics inside Kupffer{\textquoteright}s vesicle is not completely understood. We show that cells lining both the dorsal roof and the ventral floor of Kupffer{\textquoteright}s vesicle possess posteriorly pointed cilia that rotate clockwise when viewed apically. Analysis of bead movements within Kupffer{\textquoteright}s vesicle shows a net circular flow but the local flow differs in direction depending on the location within the vesicle. Histological analysis suggests that the orientation of the cells at anterior-dorsal region likely direct net flow in the vesicle. Our data suggest that the plane of the circular net flow is tilted with respect to the D-V axis, which may be converted to a local leftward flow in the anterior-dorsal region of the vesicle. },
  year = {2008},
  journal = {Dev Dyn},
  volume = {237},
  pages = {3602-12},
  month = {12/2008},
  issn = {1058-8388},
  doi = {10.1002/dvdy.21730},
  language = {eng},
}